Группа растительно-микробных взаимодействий
Group of plant-microbial interactions
Зав. группой – Федорова Елена Эриковна, в.н.с., к.б.н.
Тел.: (499) 678-54-00, внутренний: 3-05, E-mail: elenafedorova06@mail.ru
Head. of the group – Fedorova Elena Erika, Ph.D.
Tel.: (499) 678-54-00, internal: 3-05, E-mail: elenafedorova06@mail.ru
Тематика: Молекулярные механизмы регуляции внутриклеточной популяции микроорганизмов в симбиозе: мембранные контакты, везикулярный транспорт, цитоскелет, ионные переносчики.
Area of research: Molecular and cellular mechanisms of intracellular stage of symbiosis: membrane contacts, vesicular regulation of the intracellular population of microorganisms in symbiosis: membrane contacts, vesicular transport, cytoskeleton, ion carriers.
Экспертиза: клеточная биология симбиоза, иммуноцитохимия, конфокальная и электронная микроскопия.
Expertise: cell biology of symbiosis, immunocytochemistry, confocal and electron microscopy.
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| Конфокальная микроскопия. Клетка люцерны с симбиотическими бактериями и микротрубочками (иммунометка тубулина, сиреневый цвет). Confocal microscopy. Root nodule cell with symbiotic bacteria and microtubules (tubulin immunolocalization, lilac color). |
Конфокальная микроскопия, 3-D реконструкция. Инфицированная клетка люцерны. Симбиотические бактерии (красный), вакуоль (зеленый). Confocal microscopy, 3-D reconstruction. Infected alfalfa cell. Symbiotic bacteria (red), vacuole (green). |
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| Конфокальная микроскопия. Клетка корня люцерны, содержащая арбускулярную микорризу. Confocal microscopy. Alfalfa root cell containing arbuscular mycorrhizae. |
Электронная микроскопия. Мультивезикулярное тело, стрелка: иммунометка коллоидным золотом. Electron microscopy. Multivesicular body, arrow: immunolabelling with colloidal gold. |
Сотрудники группы
Федорова Елена Эриковна — руководитель группы , в.н.с., к.б.н.
Тел.: (499) 678-54-00, внутренний: 3-05, E-mail: elenafedorova06@mail.ru
Трифонова Наталья Александровна —мнс
Тел.: (499) 678-54-00, внутренний: 3-05, E-mail: natali.flow@yandex.ru
Королева Мария Игоревна, инженер, студент-магистрант,
Тел.: (499) 678-54-00, внутренний: 3-05, E-mail: koroleva181298@yandex.ru
The colleagues
Fedorova Elena Erika — group leader, ph.d.
Tel.: (499) 678-54-00, internal: 3-05, E-mail: elenafedorova06@mail.ru
Trifonova Natalia Aleksandrovna — young sci.
Tel.: (499) 678-54-00, internal: 3-05, E-mail: natali.flow@yandex.ru
Koroleva Maria Igorevna, Msc student,
Tel.: (499) 678-54-00, internal: 3-05, E-mail: koroleva181298@yandex.ru
Публикации
Publications
Fedorova EE, Coba de la Peña T, Lara-Dampier V, Trifonova NA, Kulikova O, Pueyo JJ, Lucas MM. Potassium content diminishes in infected cells of Medicago truncatula nodules due to the mislocation of channels MtAKT1 and MtSKOR/GORK. J Exp Bot. 2021 Feb 24;72(4):1336-1348. doi: 10.1093/jxb/eraa508.PMID: 33130893
Pueyo JJ, Quiñones MA, Coba de la Peña T, Fedorova EE, Lucas MM. Nitrogen and Phosphorus Interplay in Lupin Root Nodules and Cluster Roots.Front Plant Sci. 2021 Mar 3;12:644218. doi: 10.3389/fpls.2021.644218. eCollection 2021.
van Velzen R, Holmer R, Bu F, Rutten L, van Zeijl A, Liu W, Santuari L, Cao Q, Sharma T, Shen D, Roswanjaya Y, Wardhani TAK, Kalhor MS, Jansen J, van den Hoogen J, Güngör B, Hartog M, Hontelez J, Verver J, Yang WC, Schijlen E, Repin R, Schilthuizen M, Schranz ME, Heidstra R, Miyata K, Fedorova E, Kohlen W, Bisseling T, Smit S, Geurts R.
Comparative genomics of the nonlegume Parasponia reveals insights into evolution of nitrogen-fixing rhizobium symbioses. Proc Natl Acad Sci U S A. 2018 May 15;115(20):E4700-E4709. doi: 10.1073/pnas.1721395115. Epub 2018 May 1.
van Zeijl A, Wardhani TAK, Seifi Kalhor M, Rutten L, Bu F, Hartog M, Linders S, Fedorova E.E, Bisseling T, Kohlen W, Geurts R. CRISPR/Cas9-Mediated Mutagenesis of Four Putative Symbiosis Genes of the Tropical Tree Parasponia andersonii Reveals Novel Phenotypes.
Front Plant Sci. 2018 Mar 6;9:284. doi: 10.3389/fpls.2018.00284. eCollection 2018.
Yang S., Wang Q., Fedorova E., Liu J., Qin Q., Zheng Q,. Price P.A., Pan H., Wang D., Griffitts J.S., Bisseling T., Zhu H. (2017) Microsymbiont discrimination mediated by a host-secreted peptide in Medicago truncatula. Proc Natl Acad Sci U S A., 114(26), 6848-6853. doi: 10.1073/pnas.1700460114.
Gavrin A, Kulikova O, Bisseling T, Fedorova EE. (2017) Interface symbiotic membrane formation in root nodules of Medicago truncatula: the role of synaptotagmins MtSyt1, MtSyt2 and MtSyt3. Frontiers Plant Science, 8, 201. doi: 10.3389/fpls.2017.00201.
Gavrin A, Chiasson D, Ovchinnikova E, Kaiser BN, Bisseling T, Fedorova EE. (2016) VAMP721a and VAMP721d are important for pectin dynamics and release of bacteria in soybean nodules. New Phytol., 210(3) 1011-21. doi: 10.1111/nph.13837.
Gavrin A, Jansen V, Ivanov S, Bisseling T, Fedorova EE. (2015) ARP2/3-mediated actin nucleation associated with symbiosome membrane is essential for the development of symbiosomes in infected cells of medicago truncatula root nodules. Mol. Plant Microbe Interact., 28(5), 605-14. doi: 10.1094/MPMI-12-14-0402-R.
Gavrin A, Fedorova EE. (2015) Quantification of the volume and surface area of symbiosomes and vacuoles of infected cells in root nodules of Medicago truncatula. Bio-protocol, 5(22), e1665.
Moling S., Pietraszewska-Bogiel A., Postma M., Fedorova E., Hink M.A., Limpens E., Gadella T.W., Bisseling T. (2014) Nod factor receptors form heteromeric complexes and are essential for intracellular infection in medicago nodules. The Plant Cell, 26(10), 4188-99. doi: 10.1105/tpc.114.129502.
Gavrin A, Kaiser BN, Geiger D, Tyerman SD, Wen Z, Bisseling T, Fedorova EE. (2014) Adjustment of host cells for accommodation of symbiotic bacteria: vacuole defunctionalization, HOPS suppression, and TIP1g retargeting in Medicago. The Plant Cell, 26(9), 3809-22. doi: 10.1105/tpc.114.128736.
Chiasson D.M., Loughlin P.C., Mazurkiewicz D., Mohammadidehcheshmeh M., Fedorova E.E., Okamoto M., McLean E., Glass A.D., Smith S.E., Bisseling T., Tyerman S.D., Day D.A., Kaiser B.N. (2014) Soybean SAT1 (Symbiotic Ammonium Transporter 1) encodes a bHLH transcription factor involved in nodule growth and NH4+ transport. Proc Natl Acad Sci U S A., 2014, 111(13):4814-9.
Margaret I, Lucas MM, Acosta-Jurado S, Buendia-Claveria AM, Fedorova E, Hidalgo A, Rodriguez-Carvajal MA, Rodriguez-Navarro DN, Ruiz-Sainz JE, Vinardell JM. (2013) The Sinorhizobium fredii HH103 lipopolysaccharide is not only relevant at early soybean nodulation stages but also for symbiosome stability in mature nodules. PLoS One, 8(10), e74717.
Op den Camp RH, Polone E, Fedorova E, Roelofsen W, Squartini A, Op den Camp HJ, Bisseling T, Geurts R. (2012) Nonlegume Parasponia andersonii deploys a broad rhizobium host range strategy resulting in largely variable symbiotic effectiveness. Mol. Plant Microbe Interact. , 25(7), 954-63.
Ivanov S., Fedorova E.E., Limpens E., De Mita S., Genre A., Bonfante P., Bisseling T. (2012) Rhizobium-legume symbiosis shares an exocytotic pathway required for arbuscule formation. Proc Natl Acad Sci U S A. , 109(21), 8316-21.
Ovchinnikova E, Journet EP, Chabaud M, Cosson V, Ratet P, Duc G, Fedorova E, Liu W, den Camp RO, Zhukov V, Tikhonovich I, Borisov A, Bisseling T, Limpens E. (2011) IPD3 controls the formation of nitrogen-fixing symbiosomes in pea and Medicago Spp. Mol. Plant Microbe Interact. , 24(11), 1333-44. doi: 10.1094/MPMI-01-11-0013.
Ivanov S., Fedorova E., Bisseling T. (2010) Intracellular plant microbe associations; secretory pathways and the formation of perimicrobial compartments. Current Opinion in Plant Biology, 13, 1-6
Dong W., Griffitts J., Starker C., Fedorova E., Limpens E., Ivanov S., Bisseling T., Long Sh. (2010) A nodule-specific protein secretory pathway required for nitrogen-fixing symbiosis. Science, 327, 1126-1129 .
Ivashina T. V., Fedorova E. E,Ashina N. P, Kalinchuk N. A, Druzhinina T. N, Shashkov A. S, Shibaev V. N. Ksenzenko, V. N. (2010) Mutation in the pssM gene encoding ketal pyruvate transferase leads to disruption of Rhizobium leguminosarum bv. viciae-Pisum sativum symbiosis. Journal of Applied Microbiology, 109(2), 731-742
Limpens E., Ivanov S., vanEsse W., Voets G., Fedorova E., Bisseling T. (2009) Medicago N2-fixing symbiosomes acquire the endocytic identity marker Rab7 but delay the acquisition of vacuolar identity. The Plant Cell, 21, 2811-2828.
Fedorova E.E, Brown S. (2007) Cytochemical analysis of proteolytic activity and pH status of vacuoles in root nodules of Medicago truncatula. Russian J.Plant Physiology, 54(1), p25-31.
Wan X., Hontelez J., Lillo A., Guarnerio C., van de Peut D., Fedorova E., Bisseling T., Franssen H. (2007) Medicago truncatula ENOD40-1 and ENOD40-2 are both involved in nodule initiation and bacteroid development. Journal of Experimental Botany, 58(8), 2033-2041.
Smit P., Limpens E., Geurts R., Fedorova E., Dolgikh E., Gough C., Bisseling T. (2007) Medicago LYK3, an entry receptor in rhizobial Nod factor signaling. Plant Physiology, 145, 183-191.
Fedorova E. E,. de Felipe M. R, Pueyo J. J., Lucas M. M. (2007) Conformation of cytoskeletal elements during the division of infected Lupinusalbus L. nodule cells. J. Experimental Botany, 58, 2225-2236.
Gonzales-Sama, A., Coba de la Pena T.,Redondo Vela F., by Maria N., Fedorova E.E., by Lorenzo C, Hernandez-Jimenez, M.J., Pozuelo J.M., Golvano M.P., by Felipe M.R., Pueyo J.J., Fernandez-Pascual, Lucas M.M. (2006) Special characteristics of the Lupinus-Bradyrhizobiumsp symbiosis. (Lupinus). Chapter of the book, "Nitrogen fixation: fundamentals and applications",Bedmar E.J., Gonzalez J., Lluch C., Rodelas B. Sociedad Espanola de Fijacion de Nitrogeno (SEFIN), Granada, Spain,p182-193.
Limpens E., Mirabella R., Franken C., Fedorova E., Franssen H., Bisseling T., Geurts R. (2005) Formation of organelle-like N2-fixing symbiosomes in legume root nodules is controlled by DMI2. Proc Natl Acad Sci U S A, 102(29), 10375-10380.
Fedorova E., Redondo F.J., Koshiba T., Pueyo J.J., Rosario de Felipe M., Lucas M.M. (2005) Aldehyde oxidase (AO) in the root nodules of Lupinus albus and Medicago truncatula: identification of AO in meristematic and infection zones. Molecular Plant Microbe Interactions, 18(5), 405-413.
Geurts R., Fedorova E., Bisseling T. (2005) Nod factor signaling genes and their function in the early stages of Rhizobium infection. Current Opinion in Plant Biology, 8, 346-352.
Vinardell J.M., Fedorova E., Kevei Z., Cebolla A., Horvath G., Kelemen Z., Tarayre S., Roudier F., Mergaert P. Kondorosi A., Kondorosi E. (2003) Endoreduplication mediated by the anaphase-promoting complex activator CCS52 is required for symbiotic cell differentiation in Medicago truncatula nodules. The Plant Cell, 15, 2093-2105.
Roudier F., Fedorova E., Lebris M., Lecomte P., Gyorgiev J., Vaubert D., Horvath G., Kondorosi A., Kondorosi E. (2003) The Medicago species A2-type cyclin is auxin regulated and involved in meristem formation but dispensable for endoreduplication associated developmental program. Plant Physiology, 131, 1091-1103.
Goggin D.E., Lipscombe R.L., Fedorova E., Millar A.H., Mann A., Atkins C.A., Smith P.M.C. (2003) Dual intracellular localization and targeting of aminoimidazole ribonucleotide synthetase in cowpea. Plant Physiology, 131, 1033-1041
Fedorova E. Zhiznevskaya G., Kalibernaya Z., Artemenko E., Izmailov S., Gus’kov A. (2000) IAA metabolism in Phaseolus vulgaris-Rhizobium etli. Russian J. Plant Phys. , 47, 293-206.
Cermola M., Fedorova E., Tate R., Riccio A., Favre R., Patriarca E. (2000) Nodule invasion and symbiosome differentiation during Rhizobium etli-Phaseolus vulgaris symbiosis. Molecular Plant Microbe Interactions, 13(7), 733-741.
Roudier, F., Fedorova E., Gyorgyev J., Feher A., Broun S., Kondorosi A., Kondorosi E. (2000) Cell cycle function of a Medicago sativa A2-type cyclin interaction with PSTAIRE-type cycline-dependent kinase and a retinoblastoma protein. Plant J. , 23, 73-83.
Fedorova E., Thomson R., Whitehead L.,Maudoux O., Udvardi M., Day D. (1999) Localization of H-ATPases in soybean root nodules. Planta, 209, 25-32.+
Fedorova E.,R.Tate, R.Favre, M.Iaccarino, R.Defez. (1998) Ultrastrustural immunolocalization of ubiquitin/ubiquitinated proteins in mature and senescent root nodules of Phaseolus vulgaris and Vicia hirsuta. J. Submicrosc. Cytol.Pathol., , 30(1), 85-94.
Patriarca E.J., Tate R., Fedorova E., Riccio A., Defez R., Iaccarino M. (1996) Down-regulation of the Rhizobium ntr system in the determinate nodule of Phaseolus vulgaris identifies a specific developmental zone. Molecular Plant Microbe Interactions, 9, 243-251.
Fedorova E.E., Greenwood G.S., Oaks A. (1994) In situ localization of nitrate reductase in maize root. Planta, 194, 279-286